Once the player puts the note down, a loud choking sound is heard this is the indicator that Specimen 2, the first hostile enemy in the game, has appeared. However, the real scares don't begin until the player reaches Room 60, a room with a mysterious black and green puddle in the center, and a note on a table written in blood. In this section, dark maze-like rooms surrounded by abysses begin to appear, as well as out of place objects such as the Brainframe Room. Once the player reaches Room 50, which is an elevator, they are rewarded with a save point before pressing the button and continuing to the next floor. The first and only indicator that things are not as they seem are the notes that appear randomly on Specimen 1 and furniture which tell ominous accounts of the previous travelers of the mansion. In the first 50 rooms, no monsters or death traps appear, and the only jump scares are the Specimen 1s, which may cause one to completely drop their guard. Afterwards, she flies through the ceiling.
She explains the main goal of the game: to make it through 1000 rooms alive. Spooky greets the player in the first room, Room 0 her cute appearance and humorous dialogue may lead one to believe that this is going to be a harmless parody game that pokes fun at other horror game clichés. Room 0, where Spooky welcomes the Protagonist.